Surface Sample Analysis

Non-Culturable Surface Mould Analysis 

At Oceanic Laboratory Initiative (OLI), we specialize in non-culturable surface mould analysis, utilizing cutting-edge methods for precise results. Our approach adheres to the industry-standard ASTM D7658 – Standard Test Method for Direct Microscopy of Fungal Structures from Tape. 

Surface Sample Analysis  

We conduct surface analysis using adhesive tapes, with a preference for Zefon Bio Tapes or Mould Tape Slides. Alternatively, we accept clear/translucent tape placed on a microscope slide or mounted to a removable medium. Please note that OLI does not accept stickers, even for mould-related purposes. 

ASTM Standard Compliance 

We adhere to the ASTM D7658 standard, which outlines a minimum of 12 different genera of mould for analysis. This includes common water damage-related mould genus types such as Aspergillus, Cladosporium, Penicillium, Stachybotrys, Memnoniella, and Chaetomium, among others. OLI goes beyond the minimum requirements to provide a more comprehensive understanding for mould testing consultants. 

Qualitative Reporting 

To meet the semi-quantitative reporting requirements of the ASTM standard, OLI has developed a complementary process. This process helps qualitatively report on the characteristics of fungal presentations. As a result, we suggest an IICRC S520 labelling of Conditions 1, 2, and 3: 

• Condition 1: An indoor environment that may have settled spores, fungal fragments, or traces of actual growth. The identity, location, and quantity are reflective of a normal ecology for a similar indoor environment. 
• Condition 2: An indoor environment primarily contaminated with settled spores that may have originated directly or indirectly from a Condition 3 area and may have traces of actual growth. 
• Condition 3: An indoor environment contaminated with the presence of actual mould growth and associated spores. Actual growth includes growth that is active, dormant, visible, or hidden. 

Microscopic Insights 

Many characteristics of fungal growth and presentations are not visible to the naked eye. Direct microscopy allows us to visually comment on these presentations and use established industry documents to guide a defensible reporting process. Our reports are concise, enabling professional judgment while being supported by representative images that add context to observations made under the microscope. This approach ensures confidence and transparency from the laboratory to the interpreting consultant.